Regulatory

Part:BBa_K726001

Designed by: Sami Chu   Group: iGEM12_UCSF   (2012-08-08)

pLasR and RBS

LasR promoter and the RBS. The Las induction system is a quorum-sensing system from Pseudomonas aeruginosa. Insert the gene you want expressed behind this. To complete the system, you will need the LasR regulator in front of the LasR promoter. ([[1]]). The inducer of the promoter is a combination of the the autoinducer N-(3-oxododecanoyl) homoserine lactone (PAI-1), which is produced by the LasI synthetase ([[2]]), and the LasR regulator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None